Informatics Publishing Limited

C. K. Venil , Suresh S. S. Raja

Abstract

Acorus calamus commonly known as Sweet flag is a medicinal herb, effectively used in treating variety of illness. The chemicals were fractionated from rhizome of Acorus calamus and antimicrobial properties of Hydrocarbon and Beta asarone were studied. Acorus calamus were collected from Nilgiri wetland areas. The components of rhizome of Acrous calamus were separated using column chromatography. Hydrocarbon and Beta asarone parts were eluted from the column and tested for its antimicrobial assay. Antibacterial activity of fractionated beta asarone inhibited the growth of Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi and Listeria monocytogenes. There is no antibacterial activity in fractionated hydrocarbon. The determination of antimicrobial activity of Acorus calamus reveals that beta asarone is responsible for antimicrobial activity and this can be used in future for preparation of drugs.

Keywords

Acorus calamus, Anti-microbial, Beta Asarone, Hydrocarbon

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Mohammed Musthafa ¹, Alwarrappan Sankaranarayanan ², Karuppiah Ponmurugan ², Suresh S. S. Raja ³

Affiliations
1 Department of Microbiology and Immunology, College of Medicine, Prince Sattam Bin Abdulaziz University, Al Kharj– 11942, SA
2 Department of Microbiology, K.S.Rangasamy College of Arts and Science, Tiruchengode – 637 215, Tamil Nadu, IN
3 Department of Microbiology, Bharathidasan University College, Perambalur, Tamil Nadu, IN

Abstract

The principal aim of the present investigation was the isolation, screening and testing of the antibacterial activity of the Loligo duvauceli extract derived from antimicrobial peptides (AMPs). A live marine animal specimen of L. duvaucelli (Indian squid) was collected from the trawling net of fishermen at Puthiyappa fishing harbor, Calicut, South West Coast of India. Ninty five percentages of methanol and ethanol were used to prepare the crude extract of marine invertebrate. Using the disc diffusion method, the antibacterial activity against the selected bacterial pathogens isolated from pus infection was studied. The antibacterial properties containing peptides were subjected to High Performance Thin Layer Chromatography (HPTLC) and partially purified. The HPTLC analysis of crude extracts ensured the presence of six different AMPs. Further, hemolytic assay, proteinase K treatment and heat treatment were undertaken to confirm the presence of proteins in the partially purified extracts (AMPs). The crude extract of L. duvaucelli showed antibacterial properties against all the tested clinical pathogens. The AMPs isolated from marine animals possessed antimicrobial properties. The HPTLC analysis of extracts revealed the presence of six different bioactive peptides and two AMPs among them exhibited effective antibacterial activities.

Keywords

Loligo duvauceli, Indian Squid, Antimicrobial Peptides, Pyogenic Infection.

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